Vincent Price
Professor Ratigan
Barrie Ingham
Basil of Baker Street / Bartholomew
Val Bettin
Dr. David Q. Dawson
Candy Candido
Fidget
Diana Chesney
Mrs. Judson
Eve Brenner
The Mouse Queen
Alan Young
Hiram Flaversham
Melissa Manchester
Miss Kitty Mouse
Brian Cummings
Susanne Pollatschek
Olivia Flaversham (voice)
Basil Rathbone
Sherlock Holmes (voice)
Ellen Fitzhugh
Bar Maid (voice)
Walker Edmiston
Citizen (voice)
Wayne Allwine
Thug Guard (voice)
Tony Anselmo
Thug Guard (voice)
Laurie Main
Dr. Watson (archive sound)
Shani Wallis
Lady Mouse (voice)